RNA-Seq Reproducibility Assessment of the Sequencing Quality Control Project
نویسندگان
چکیده
منابع مشابه
A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequencing Quality Control Consortium
performance of RNA-seq across laboratories and to test different sequencing platforms and data analysis pipelines. Here we report a multisite, cross-platform analysis of RNA-seq measurement performance in a controlled setting. We sequenced commercially available reference RNA samples spiked with synthetic RNA from the External RNA Control Consortium. Two distinct samples were assessed individua...
متن کاملRNA-seq Data Employed in The Sequencing Quality Control (SEQC) Project
This vignette briefly describes the content in the seqc package. The seqc package provides a series of data frames derived from the SEQC project between 2011 to 2014. Three types of data frames are included in this package: RNA-seq read count tables, junction tables and an additional gene intensity table generated by using the TaqMan RT-PCR technology. All the data frames are ready to use in th...
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MOTIVATION RNA-seq has been extensively used for transcriptome study. Quality control (QC) is critical to ensure that RNA-seq data are of high quality and suitable for subsequent analyses. However, QC is a time-consuming and complex task, due to the massive size and versatile nature of RNA-seq data. Therefore, a convenient and comprehensive QC tool to assess RNA-seq quality is sorely needed. ...
متن کاملA pipeline for RNA-seq data processing and quality assessment
SUMMARY We present an R based pipeline, ArrayExpressHTS, for pre-processing, expression estimation and data quality assessment of high-throughput sequencing transcriptional profiling (RNA-seq) datasets. The pipeline starts from raw sequence files and produces standard Bioconductor R objects containing gene or transcript measurements for downstream analysis along with web reports for data qualit...
متن کاملQuality assessment and control of tissue specific RNA-seq libraries of Drosophila transgenic RNAi models
RNA-sequencing (RNA-seq) is rapidly emerging as the technology of choice for whole-transcriptome studies. However, RNA-seq is not a bias free technique. It requires large amounts of RNA and library preparation can introduce multiple artifacts, compounded by problems from later stages in the process. Nevertheless, RNA-seq is increasingly used in multiple studies, including the characterization o...
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ژورنال
عنوان ژورنال: Cancer Informatics
سال: 2020
ISSN: 1176-9351,1176-9351
DOI: 10.1177/1176935120922498